| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Wolk, Isabel H (10)
|
NCNCAC |
39.27 |
39.44 |
0.17 |
| 2 |
Light, Chloe C (9)
|
NCMOR |
46.52 |
43.06 |
-3.46 |
| 3 |
Giovanello, Aurora J (10)
|
NCNCAC |
51.21 |
45.03 |
-6.18 |
| 4 |
Emrich, Abigail G (10)
|
NCNCAC |
50.48 |
45.59 |
-4.89 |
| 5 |
Thomas, Arianna M (10)
|
NCNCAC |
47.98 |
47.14 |
-0.84 |
| 6 |
Jang, Julia Y (10)
|
NCNCAC |
46.88 |
47.58 |
0.70 |
| 7 |
Muminovic, Anya S (10)
|
VALY |
49.15 |
48.07 |
-1.08 |
| 8 |
Napravnik, Sophia (10)
|
NCNCAC |
49.01 |
48.90 |
-0.11 |
| 9 |
Whitlock, Devon B (9)
|
NCMOR |
54.17 |
50.69 |
-3.48 |
| 10 |
Collins, Daisy B (9)
|
NCNCAC |
56.35 |
51.29 |
-5.06 |
| 11 |
Hill, Eleanor E (10)
|
NCNCAC |
55.46 |
51.71 |
-3.75 |
| 12 |
Taub, Anna J (10)
|
NCNCAC |
54.35 |
51.89 |
-2.46 |
| 13 |
Collins, Mackenzie K (10)
|
NCNCAC |
54.39 |
52.17 |
-2.22 |
| 14 |
Brown, Shelby E (10)
|
NCSSS |
54.43 |
55.65 |
1.22 |
| 15 |
Li, Joie N (10)
|
NCNCAC |
1:04.11 |
59.48 |
-4.63 |
| 16 |
Biswas, Emma A (9)
|
NCNCAC |
1:04.56 |
1:02.05 |
-2.51 |
| 17 |
Li, Angela (10)
|
NCNCAC |
1:07.76 |
1:03.58 |
-4.18 |
| 18 |
Burton, Chloe G (9)
|
NCNCAC |
1:15.99 |
1:08.19 |
-7.80 |
| 19 |
Lan, Michelle H (10)
|
NCNCAC |
1:14.95 |
1:13.72 |
-1.23 |
| 20 |
McCowen, Sarah E (7)
|
NCNCAC |
1:30.99 |
1:17.59 |
-13.40 |
|
Kim, Kenzie A (10)
|
NCNCAC |
47.86 |
DQ |
- |